Earlier we have reported in vitro antioxidant and DNA protection ability of the methanol extract and its derived fractions. Roots are cooked with maize flour and used for the treatment of bone fractures. To cope with such reactive species and the clinical disorders, it is essential to obtain dietary antioxidants which counter measure the excessive generation of free radicals 3, 4. Histopathological investigation of testes revealed the damaging action of CCl4 in rat. Various reports revealed that carbon tetrachloride (CCl4) a well-established hepatotoxin also causes injuries in testes 2, 3.
The final purity of the Leydig cells isolated this way consistently was about 80%. The final purity of the Leydig cells, determined by staining the cells for 3β-HSD activity (19), was consistently about 95%. For in vitro experiments, the dissociated cells were purified by centrifugal elutriation and Percoll gradient centrifugation (18). Some of the cells were frozen in liquid nitrogen immediately after purification to assay their GSH content (see below).
Other than eating lots of dark green vegetables, there a number of things you can incorporate into your day-to-day routine to optimize glutathione levels. The results showed that T production had halved – a clear indication that glutathione regulated testosterone production. In one study , 468 men were given a selenium supplement, which after 26 weeks was seen to increase testosterone levels, improve sperm motility and increase semen parameters – all in infertile subjects. Not only does glutathione fight toxic invaders, it also supports testosterone production. What is important here is that glutathione also plays an important role regulating testosterone production.
It is involved in a wide range of processes including detoxification, antioxidant protection, and cellular metabolism. Thus, although the in vitro studies presented herein argue that BSO directly affects Leydig cell function, we cannot rule out the possibility of additional indirect effects in vivo. It is possible, therefore, that reduced GSH in organs other than the testes might have an indirect effect on Leydig cell function. These in vitro results led us to determine whether there also is an effect of BSO treatment in vivo on Leydig cell steroidogenic function. BSO supplementation in the culture medium significantly depleted the GSH pool in cultured Leydig cells by more than 70% in 48 h, and this was accompanied by significantly reduced steroidogenic function.
Rats were housed in the animal facilities of the Johns Hopkins Medical Institutions, under conditions of controlled light (14 h light, 10 h dark) and temperature (22 C) and with free access to rat chow and water. Young (4 months old) and aged (24 months old) male Brown Norway rats were obtained through the National Institute on Aging, supplied by Harlan Sprague Dawley Inc. (Indianapolis, IN). All steroids, including pregnenolone, progesterone, 17-hydroxyprogesterone, androstenedione, and testosterone were from Steraloids (Newport, RI).

جنسیت: زن

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